Thursday, 29 March 2018

Containment policy

There is a strict, enforced containment policy around restricted and unwanted micro-organisms.  The capital letter "Q" means restricted (under permit), while "U' means unwanted.  There are three containment facilities at Auckland with different degrees of containment.  Containment 2, Newhook Laboratory, is the most restricted, and all work on restricted and unwanted micro-organims must be conducted there.

As part of the kauri die back project, it is necessary to work with the pathogen, Phytopathora agathidicida (PTA). It has been identified as a casual agent in attacking New Zealand kauri (Agathis australis), but scientists do not know exactly how it is attacking.  Kauri die back disease causes a yellowing, thinning canopy and sudden canopy collapse.  The kauri die-back project is looking at the potential for a biochemical control of  the disease.

The pathogen must be contained very strictly, as noted in the Containment policy document.  As Isabel and I are working with this fungi, it was necessary for Megan to give us an induction on all aspects of containment and for us to study for and sit a written test.  We needed to achieve a pass rate of 80% to be able to work with on the project.  We must always work in PC2 under the ultra-violet hood; the specimen must not be removed from PC2, and all associated waste must be put in a specific waste container, and then auto-claved and then incinerated.  



Phytopathora agathidicida was given the permit number Q261, and the hard copy permit
was kept in the permit folder alongside the containment facility manual.


The agathidicida sample is kept under very strict conditions and must
stay in the PC2 facility at all times, as per the permit instructions.