A day after I had plated my first culture, I looked at them with Megan. In a natural environment of 25 degrees C they should grow overnight. You can see from this initial growth, that I have spread the culture correctly on this plate. Using a loop (a small plastic rod with a loop at one end), I had spread bacteria in solution as follows: first I spread a concentrated solution in four closely aligned strokes, I then turned the petri dish in a clockwise direction and 'caught' the edge of the concentrated solution to make another four lines which were less concentrated. Lastly I turned the petri dish around in a clockwise direction again, "caught' the edge of this less concentrated solution and made a final squiggle isolating the culture even more. This pattern of application is a universal way of plating cultures.
