Tuesday, 10 April 2018

Inhibition assays

I prepared inhibition assays for the kauri die-back project by taking little plugs of the fungal subcultures, two at a time,  so that they oppose each other on the petri dish.  This is a way to observe which fungus grows the strongest and thus inhibits the other's growth.  Over time, it will provide valuable data as scientists work towards a potential biocontrol of the disease.


Instructions from Marj written in my notebook as diagrams for clarity.

It is necessary to work in the PC2 hood when working on Q cultures.  It has a 20 minute cycle of ultra violet light to sterilise surfaces before and after work is carried out.