Sub cultures in liquid media

Sub cultures of the fungi used in the kauri die back project - Trichoderma, Illyonectra and Fortini, and Agathidicida - were dropped into flasks of PDB and allowed to grow.  They were incubated at 20 degrees on the 'juggler'  ( a device that keeps flasks moving in a constant smooth fashion).  To give them sufficient time to incubate, Marg instructed me to check on them in about a week.

After four days, you can see the cloudiness indicating fungal growth.

Some cultures grow more slowly, and need more time.