Liquid cultures

 On Day 12, all the liquid cultures of Illyonederma,  Trichoherma and Fortini had grown sufficiently.  Maj directed that the 'lawn' cultures of Phytophthora Agathicicida  grown earlier (9 March and 16 March) are to be cultured with the concentrated liquid culture.

The cloudiness of the liquid indicates growth.

Using the centrifuge, I watched Maj layer the liquid cultures so that the concentrate gravitated to the bottom of the tube, and she was able to discard the clear liquid at the top.  

Loading up the centrifuge - there were three samples, so a tube of water was used for balance.

The three concentrated samples were cultured on to PTA lawn, and incubated at 18 degrees C.  After observing Maj, I was able to prepare one but it was difficult to do, as the concentrated cultures were quite viscous.  These cultures will be incubated at 18 degrees C and the growth monitored and recorded digitally as before.