Making liquid media

The fungal subcultures for the kauri die back need to be grown in liquid culture, so first I had to make Potato Detrose Broth.  This has the same nutritional content as Potato Dextrose Agar (PDA) but is in a different form.

I use my lab notebook to write down exactly what I have to do, so that I have a reference for later and can look things up, a little like CBS Maths modelling books

You need to mix up the liquid media very thoroughly.

Pouring the liquid media into flasks.

The media must be sterilised in the autoclave.  As a check, the stripes of the tape go black after being autoclaved.