Kombucha - isolating bacteria

When checking Kombucha subcultures on Day 4, Megan confirmed that that some of the cultures were pure isolates.  I subbed these again twice - one for ID through genetic sequencing and the other for the ICMP collection (if the sequencing confirmed its status as a new bacteria or yeast).  A wonderfully exciting moment in the lab!

Under Megan's guidance, I made wet slides to look at some of the bacteria and yeast in the Kombucha samples under 40x magnification, to see the differences in shape and size between the two cultures.  I found focusing very difficult, lots of practice required.  

This image using the lab camera shows a pure isolate - these images are kept on file.

This shows contamination, so it was subbed again.